Background and objective: Self-esteem is the most important factor in the spiritual growth and has an important effect on the mental process, emotion, aptitude, values and goal of person. Sport makes the person feel self sufficient and increase his/her efficiency. The sufficiency and efficiency can cause a feel in control of life and enhance self-esteem. The aim of this study was to determine the effect of regular exercise program on self esteem of nursing students.Methods: This clinical trial study was done on 80 students who lived in a dormitory. They were randomly divided into control and case groups. In case group, regular exercise program was done for 8 weeks and in control group no interference occurred. The amount of self esteem was measured before and after study and compared to each other. Coppersmith self esteem scale was used in this study.Findings: The mean of nursing students’ self esteem in case group before and after regular exercise program was 93.1±8.2 and 111.4±8.1, respectively and in control group before interference was 91.2±7.9 and in the end of study was 92.4±6.3. Also, the results showed that after interference, the difference in the average for self esteem scores in control and case groups was -1.5±3.8 and 9.9±4, respectively. The differences in the average of self esteem scores in case and control groups were statistically significant (p<0.001(.Conclusion: the results showed that regular exercise program can increase student’s self esteem. So, considering fulfillment facility, benefits of physical activity and no serious side effect, using sport as a factor for enhancing self esteem and physical and mental health of students is suggested.